BBa_K176161

BBa_K176161 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K176161
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Zongxiao He, Hao Jiang
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:03

PoPS->RBS(weak)+ccdB-LVA->PoPS



    • Details

    Types
    DnaRegion

    Roles
    Translational_Unit

    engineered_region

    Sequences BBa_K176161_sequence (Version 1)

    Description

    RBS(weak) + ccdB-LVA

    Notes

    weak RBS + ccdB-LVA coding

    Source

    PCR from K176060.

    Forward Primer: VF2(G00100)

    Reverse Primer: GTTT ACTAGT A TTATTA AGC TAC TAA AGC GTA GTT TTC GTC GTT TGC AGC tattccccagaacatcaggtta

    Digest with EcoRI and SpeI, ligate into pSB1A3.

    Access Instance Definition
    public
    public
    		BBa_B0031
    BBa_K176157
    		BBa_B0031
    ccdB-LVA

    Sequence Annotation Location Component / Role(s)
    BBa_B0031
    BBa_K176157
    		1,14
    21,362
    		BBa_B0031
    ccdB-LVA

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K176161/1
     

    Attachments

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