Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Zongxiao He, Hao Jiang
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:03
PoPS->RBS(weak)+lacZalpha-ccdB-LVA->PoPS
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K176162_sequence (Version 1) |
Description
RBS(weak) + lacZalpha-ccdB-LVANotes
weak RBS + lacZalpha-ccdB-LVA codingSource
PCR from K176061.Forward Primer: VF2(G00100)
Reverse Primer: GTTT ACTAGT A TTATTA AGC TAC TAA AGC GTA GTT TTC GTC GTT TGC AGC tattccccagaacatcaggtta
Digest with EcoRI and SpeI, ligate into pSB1A3.
| Access | Instance | Definition |
| public public | BBa_B0031 BBa_K176158 | BBa_B0031 lacZa-ccdB |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0031 BBa_K176158 | 1,14 21,533 | BBa_B0031 lacZa-ccdB |