Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: naseeb saida
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 08:57:51
P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS eyfp ter ter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1767009_sequence (Version 1) |
Description
We designed and built a kill switch mechanism that based on the activation of a lethal gene downstream of an inducible promoter that can be activated over time.
In order to understand our system we started to test the first part of our genetic circuit , to test it we built the first part ( k1767001 ) driving expression of florescence protein .
The here presented BioBrick consists of a constitutive promoter(I14032) driving expression of LuxR followed by a Lux promoter (R0062) , driving expression of YFP.
The idea is to retrieve information about the expression of a fluorescent reporter downstream of the pLuxR promoter in absence and increasing concentrations of AHL.
The scheme below explains how this BioBrick works :
Notes
noneSource
iGEM registry| Access | Instance | Definition |
| public public | BBa_K1767001 BBa_I13003 | BBa_K1767001 BBa_I13003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1767001 BBa_I13003 | 1,1818 1827,2705 | BBa_K1767001 BBa_I13003 |