BBa_K1773019

BBa_K1773019 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1773019
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sarunas Tumas
Date created: 2015-08-25 11:00:00
Date modified: 2015-09-12 07:15:36

Cas3 gene with pLux/cI right promoter and strong RBS



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Generator

    Sequences BBa_K1773019_sequence (Version 1)

    Description

    Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is an helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and strong RBS site. In the I-F type CRISPR-Cas system Cascade complex distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction.

    Notes

    No.

    Source

    I-F type CRISPR-Cas system was cloned from Aggregatibacter actinomycetemcomitans D7-S strand.

    Access Instance Definition
    public
    public
    		BBa_K1773016
    BBa_K1773003
    		BBa_K1773016
    BBa_K1773003

    Sequence Annotation Location Component / Role(s)
    BBa_K1773016
    BBa_K1773003
    		1,93
    100,3385
    		BBa_K1773016
    BBa_K1773003

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1773019/1
     

    Attachments

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