Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM15_Tsinghua
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-18 07:05:24
dCas9-Long Linker (Gly-Gly-Ser-Gly)x3-Bxb1 gp35
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1797015_sequence (Version 1) |
Description
In this part, we fuse dCas9(BBa_K1797006) and catalytic domain of Bxb1 gp35(BBa_K907000) with a long linker(BBa_K243006). Bxb1 gp35 is a site-specific recombinase. Due to its specific recognition site, the use of recombinase is limited. To solve this problem, we fuse its catalytic domain with dCas9. Then where the Bxb1 gp35 is going to function is decided by gRNA.Notes
No.Source
No.| Access | Instance | Definition |
| public public public | BBa_K1797006 BBa_K243006 BBa_K907000 | BBa_K1797006 LongLinker Bxb1_Int |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1797006 BBa_K243006 BBa_K907000 | 1,4107 4116,4151 4158,5660 | BBa_K1797006 LongLinker Bxb1_Int |