BBa_K1797015

BBa_K1797015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1797015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM15_Tsinghua

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-18 07:05:24

dCas9-Long Linker (Gly-Gly-Ser-Gly)x3-Bxb1 gp35

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1797015_sequence (Version 1)

Description

In this part, we fuse dCas9(BBa_K1797006) and catalytic domain of Bxb1 gp35(BBa_K907000) with a long linker(BBa_K243006). Bxb1 gp35 is a site-specific recombinase. Due to its specific recognition site, the use of recombinase is limited. To solve this problem, we fuse its catalytic domain with dCas9. Then where the Bxb1 gp35 is going to function is decided by gRNA.

Notes

No.

Source

No.

Components

Access	Instance	Definition
public	BBa_K1797006	BBa_K1797006
public	BBa_K243006	LongLinker
public	BBa_K907000	Bxb1_Int

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1797006	1,4107	BBa_K1797006
BBa_K243006	4116,4151	LongLinker
BBa_K907000	4158,5660	Bxb1_Int

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1797015/1

Member of these Collections

• iGEM Parts Registry