Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Morgan
Date created: 2015-08-02 11:00:00
Date modified: 2015-08-03 01:54:09
Const. Promoter-RBS-BclA-GaAFP-DT
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1809002_sequence (Version 1) |
Description
This part contains the constitutive promoter BBa_J23100, an RBS, the cell surface expression tag BclA, Glaciozyma antarctica antifreeze protein (GaAFP), and a double terminator. It encodes an antifreeze protein that attaches to the cell surface.Notes
Spliced together a cell surface expression tag and the antifreeze protein to test cell surface activity.Source
Composite of parts BBa_K1423005 and BBa_K1809004 without the YFP tag.| Access | Instance | Definition |
| public public public public public public | BBa_J23100 BBa_B0034 BBa_K1423000 BBa_K1809000 BBa_B0010 BBa_B0012 | BBa_J23100 BBa_B0034 BBa_K1423000 BBa_K1809000 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K1423000 BBa_K1809000 BBa_B0010 BBa_B0012 | 1,35 44,55 64,126 133,663 672,751 760,800 | BBa_J23100 BBa_B0034 BBa_K1423000 BBa_K1809000 BBa_B0010 BBa_B0012 |