Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samuel Keating
Date created: 2015-08-25 11:00:00
Date modified: 2015-08-26 01:03:50
CUP1 metallothionein fused to LamB with promoter and RBS
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1811666_sequence (Version 1) |
Description
LamB is a membrane channel that transports maltose and maltodextrins as well as the receptor for phage lambda. Metallothionein is a protein that sequesters 11 copper atoms and detoxifies radicals produced by copper. This part is a composite of the CUP1 LamB fusion (BBa_K1811333), a strong constituitive promoter (BBa_J23100), and an RBS (BBa_B0034).Notes
The stop codon of CUP1 was removed before being inserted into LamB. In making a G Block for this part to be synthesized, a 20 bp overlap with the plasmid pSB1C3 needed to be added to the ends of the sequence. This needed to be done to allow Gibson Assembly to yield our completed plasmid containing this part in pSB1C3.Source
LamB comes from NCBI ID 948548. CUP1 comes from NCBI ID 856450.| Access | Instance | Definition |
| public public public | BBa_J23100 BBa_B0034 BBa_K1811333 | BBa_J23100 BBa_B0034 BBa_K1811333 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K1811333 | 1,35 44,55 62,1585 | BBa_J23100 BBa_B0034 BBa_K1811333 |