BBa_K1819008

BBa_K1819008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1819008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: La??s Ribovski

Date created: 2015-09-15 11:00:00

Date modified: 2015-09-16 06:09:11

Promoter test circuit to analyze the kill switch efficiency

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1819008_sequence (Version 1)

Description

BBa_K1819008 construction indicates lactose promoter (pLac) efficiency to control a TetR promoter inhibited by TetR protein. This circuit was used to verify a kill switch mechanism (See more details in Brasil-USP team wiki)

In the absence of pLac inductor (Lactose or IPTG), RFP will be constitutively expressed under TetR promoter control. If inducer is added, TetR promoter is repressed and, consequently, RFP expression ceases and GFP expression starts.

Notes

Two different fluorescent protein, GFP and RFP, were chosen to detect expression variation considering their emission and excitation wavelength to avoid spectra superposition.

Source

This part results from the assembly of BBa_R0010, BBa_B0034, BBa_E0040, BBa_P0440, BBa_R0040 and BBa_I13507.

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034
public	BBa_E0040	GFP
public	BBa_P0440	BBa_P0440
public	BBa_R0040	p(tetR)
public	BBa_I13507	BBa_I13507

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0034	209,220	BBa_B0034
BBa_E0040	227,946	GFP
BBa_P0440	955,1794	BBa_P0440
BBa_R0040	1803,1856	p(tetR)
BBa_I13507	1865,2725	BBa_I13507

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1819008/1

Member of these Collections

• iGEM Parts Registry