Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenbo Xu, Hao Wu
Date created: 2015-09-03 11:00:00
Date modified: 2015-09-17 07:16:43
Constitutive Promoter J23119 + RNA Thermometer A1
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1824000_sequence (Version 1) |
Description
This was a composite regulatory part that consisting of constitutive promoter J23119 and RNA Thermometer A1 for temperature induced gene expression at 42 degrees. A1 was derived from ROSE family (ROSE stands for Repression Of heat Shock gene Expression), first discovered in the 5′ untranslated region of rhizobial heat shock genes (Nocker, 2001). The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination was compatible with the assembly method that XJTLU-CHINA promoted.Notes
The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. it is important to make sure that those redundant sequences do not interact with RNA thermometers.Source
J23119 was from the Registry, and A1 was from BIT-CHINA.| Access | Instance | Definition |
| public public | BBa_J23119 BBa_K1824556 | BBa_J23119 BBa_K1824556 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23119 BBa_K1824556 | 1,35 36,77 | BBa_J23119 BBa_K1824556 |