Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenbo Xu
Date created: 2015-09-05 11:00:00
Date modified: 2015-09-06 06:01:20
Consensus Promoter T7 + RNA Thermometer FourU
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1824007_sequence (Version 1) |
Description
This is a composite regulatory part that consisting of T7 promoter and RNA Thermometer FourU K1824561 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.Notes
The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequenceSource
FourU was previously submitted by TuDelft 2008 and T7 promoter was from registry| Access | Instance | Definition |
| public public | BBa_J64997 BBa_K1824561 | BBa_J64997 BBa_K1824561 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J64997 BBa_K1824561 | 1,19 20,85 | BBa_J64997 BBa_K1824561 |