Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenbo Xu
Date created: 2015-09-05 11:00:00
Date modified: 2015-09-06 06:33:57
Consensus Promoter T7 + RNA Thermometer U6
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1824008_sequence (Version 1) |
Description
This is a composite regulatory part that consisting of T7 promoter BBa_I13453 and RNA Thermometer U6 BBa_K1824564 for temperature induced gene expression at 37 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.Notes
The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequenceSource
T7 promoter and U6 were from registry.| Access | Instance | Definition |
| public public | BBa_I13453 BBa_K1824564 | BBa_I13453 BBa_K1824564 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13453 BBa_K1824564 | 1,130 131,199 | BBa_I13453 BBa_K1824564 |