pMH

BBa_K1830000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1830000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mathieu Husser
Date created: 2015-08-05 11:00:00
Date modified: 2015-10-09 10:24:45

pET15BB



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_K1830000_sequence (Version 1)

Description

IPTG inducible E. Coli Expression vector with RCF25 compatible MCS

Source

MH

Sequence Annotation Location Component / Role(s)
T7
LacO
ATG
6X His tag
Thrombin
NgoMIV
SpeI
T7
LacI gene
pSB1C3 backbone
CAT gene
TAG
NdeI
BamHI
1,20
20,42
88,90
100,117
127,144
151,156
213,218
241,293
2327,3529
291,2033
1435,2092
241,243
145,150
162,167
promoter feature/promoter
feature/binding non_covalent_binding_site
start_codon feature/start
tag feature/tag
tag feature/tag
engineered_region feature/BioBrick
engineered_region feature/BioBrick
feature/stem_loop stem_loop
feature/cds CDS
sequence_feature feature/dna
feature/cds CDS
stop_codon feature/stop
sequence_feature feature/misc
feature/misc sequence_feature
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1830000/1