BBa_K1845000

BBa_K1845000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1845000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Edoardo Gianni
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 07:19:13

RFP Reporter/Screening plasmid intermediate



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Reporter

    Sequences BBa_K1845000_sequence (Version 1)

    Description

    This part is an improvement on BBa_I13507, which has been distributed with the incorrect sequence.

    It consists in a Reporter Coding Regions of an RBS (B0034) an mRFP coding sequence (E1010), a barcode, and B0015 terminator. It can be used downstream of a promoter to test it's efficacy. We hope that this part will be used in place of the incorrect twin BBa_I13507

    Notes

    The RFP was amplified using the following primers:
    - GTTCTTCGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCC (F)
    - GACTGCAGCGGCCGCTACTAGTA ??

    The part was assembled using SLiCE (PPY) assembly.

    Source

    This part was amplified from the J04450 standard RFP Generator contained in a pSB1K3 plasmid from the 2014 iGEM distribution kit.

    igem#sampleStatus
    In stock
    igem#status
    Planning
     
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1845000/1
     

    Attachments

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