Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yusuke Banno
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-17 08:36:19
mRNA circularize efficiently generator (inside complementarityⅡ
)
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1859025_sequence (Version 1) |
Description
This device is all-in-one mRNA circularization device. If you transfer this device into E. coli, you can get a protein (its sequence is ???GGSGGS - amino acid derived from RBS sequence - HisRFP ??? HHHHHH - amino acid derived from ribozyme sequence ??? GGSGGS - amino acid derived from RBS sequence -???). Two types of the sequence derived from td gene are complementarity sequences with each other. So we are hoping for an improvement of the efficiency of circularization.GGSGGS-HHHHHH
Notes
This part includes complementary sequences.Source
T4 phage| Access | Instance | Definition |
| public public public public public public | BBa_R0010 BBa_K1859001 BBa_B0034 BBa_K1332002 BBa_K1859006 BBa_B0015 | LacI BBa_K1859001 BBa_B0034 BBa_K1332002 BBa_K1859006 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_K1859001 BBa_B0034 BBa_K1332002 BBa_K1859006 BBa_B0015 | 1,200 209,414 423,434 443,1145 1154,1286 1295,1423 | LacI BBa_K1859001 BBa_B0034 BBa_K1332002 BBa_K1859006 BBa_B0015 |