Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yusuke Banno
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-17 08:27:44
mRNA circularize efficiently generator (outside complementarity)
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1859026_sequence (Version 1) |
Description
This device is all-in-one mRNA circularization device. If you transfer this device into E. coli, you can get a protein (its sequence is ???- amino acid derived from RBS sequence - HisRFP - amino acid derived from ribozyme sequence ???amino acid derived from RBS sequence -???). Two types of the sequence derived from td gene (inserted outside the circularized area) are complementarity sequences with each other. So we are hoping for an improvement of the efficiency of circularization.Notes
This part includes complementary sequences.Source
T4 phage| Access | Instance | Definition |
| public public public public public public | BBa_R0010 BBa_K1859015 BBa_B0034 BBa_K1332002 BBa_K1859016 BBa_B0015 | LacI BBa_K1859015 BBa_B0034 BBa_K1332002 BBa_K1859016 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_K1859015 BBa_B0034 BBa_K1332002 BBa_K1859016 BBa_B0015 | 1,200 209,414 423,434 443,1145 1154,1327 1336,1464 | LacI BBa_K1859015 BBa_B0034 BBa_K1332002 BBa_K1859016 BBa_B0015 |