Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lejian Jiang
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 11:12:47
broken Ptet-cph8
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1886006_sequence (Version 1) |
Description
This circuit is composed of the constitutive promoter -- broken Ptet and membrane protein cph8. Under dark conditions, protein cph8 could transfer its phosphoryl group to intracellular protein ompR (ompR is native protein carried by E.coli), and this process could be inhibited by 650-nm light.Notes
There was a SpeI site in the sequence encoding protein cph8, so we designed a silent mutation.Source
xxx| Access | Instance | Definition |
| public public public public | BBa_K1886017 BBa_B0034 BBa_I15010 BBa_B0012 | BBa_K1886017 BBa_B0034 BBa_I15010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1886017 BBa_B0034 BBa_I15010 BBa_B0012 | 1,49 58,69 76,2338 2347,2387 | BBa_K1886017 BBa_B0034 BBa_I15010 BBa_B0012 |