Types | DnaRegion
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Roles | engineered_region
Generator
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Sequences | BBa_K188774_sequence (Version 1)
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Description
To inhibit transcription of the LacI promoter (pLacl, BBa_R0010), we have to encode LacI protein (BBa_C0012). LacI protein can bind to pLacI and repress the expression, but lactose, which repress lacI binding, act as a switch opening the expression. Therefore, the inter-regulation could be a timer device, so LacI is a key component of first device. The second gene is the LuxR, which is a membrane receptor, takes external AHL compound to pass down the signals. We use the LuxR as our bacteria-sensor to sense other bacteria in the environment. For selecting other parts, we choose one kind of constitutive promoter family(BBa_J23106) as the promoter, and the expression amount of this promoter is moderate comparing others in the family. Furthermore, we use the commom ribosome-binding site(BBa_B0034) and the terminator(BBa_B0014), which is a special double terminator, to ensure the transcription would exactly stop.
Notes
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Source
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