BBa_K188774

BBa_K188774 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K188774
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pei-Chuan Chu
Date created: 2009-09-23 11:00:00
Date modified: 2015-05-08 01:11:13

no.1



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K188774_sequence (Version 1)

Description

To inhibit transcription of the LacI promoter (pLacl, BBa_R0010), we have to encode LacI protein (BBa_C0012). LacI protein can bind to pLacI and repress the expression, but lactose, which repress lacI binding, act as a switch opening the expression. Therefore, the inter-regulation could be a timer device, so LacI is a key component of first device. The second gene is the LuxR, which is a membrane receptor, takes external AHL compound to pass down the signals. We use the LuxR as our bacteria-sensor to sense other bacteria in the environment. For selecting other parts, we choose one kind of constitutive promoter family(BBa_J23106) as the promoter, and the expression amount of this promoter is moderate comparing others in the family. Furthermore, we use the commom ribosome-binding site(BBa_B0034) and the terminator(BBa_B0014), which is a special double terminator, to ensure the transcription would exactly stop.

Notes

zxc

Source

zxc

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K188774/1