Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pei-Chuan Chu
Date created: 2009-09-23 11:00:00
Date modified: 2015-05-08 01:11:13
no.6
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K188779_sequence (Version 1) |
Description
In the final device in our sequence, we encode the promoter (BBa_K145150), which can be induced by the LuxR-AHL complex, and inhibited by protein cIIp22 (produced in previous device). When LuxI is produced ( produced when lactose consumed in previous device), it can stimulate the formation of AHL, which can be regarded as outer bacteria invasion. Therefore, the LuxR-AHL complex can be used as signs of timer and detecting outer bacteria. Finally, the gene mRFP1(BBa_E1010) is encoded, which can illustrate red fluorescence, let we know that the time is up or outer bacteria have invaded. Furthermore, we use the common RBS(BBa_B0034) and the double terminator(BBa_B0014) to ensure the transcription would exactly stop. In our experiments, we only encode mRFP1 without gene ccdB, because we only interest in how the promoter and gene mRFP1 demonstrate. Find out the appropriate concentration of LuxR ???AHL complex that induce the promoter and the degree of the red fluorescence intensity.Notes
zxcSource
zxc| Access | Instance | Definition |
| public public public public | BBa_K145150 BBa_B0034 BBa_E1010 BBa_B0014 | BBa_K145150 BBa_B0034 mRFP1 BBa_B0014 |