BBa_K1898150

BBa_K1898150 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1898150

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Fiona Tsai

Date created: 2016-10-09 11:00:00

Date modified: 2016-10-19 09:44:01

Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1898150_sequence (Version 1)

Description

BBa_K880005 consists of strong promoter and strong rbs to maximize protein production. This construct codes for ch25h, a catalyst for the conversion of cholesterol to 25 hydroxycholesterol, and 10x histidine tag for protein purification. BBa__B0015 is a double terminator used to stop transcription.

Notes

We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA.

Source

this DNA is synthesized by IDT.

Components

Access	Instance	Definition
public	BBa_K880005	BBa_K880005
public	BBa_K1898100	BBa_K1898100
public	BBa_S05336	BBa_S05336

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K880005	1,55	BBa_K880005
BBa_K1898100	62,877	BBa_K1898100
BBa_S05336	886,1058	BBa_S05336

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1898150/1

Member of these Collections

• iGEM Parts Registry