BBa_K190038

BBa_K190038 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K190038

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nienke Kuipers

Date created: 2009-09-07 11:00:00

Date modified: 2015-05-08 01:11:15

Arsenic accumulation device (lactose promoter + fMT + GlpF)

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K190038_sequence (Version 1)

Description

A device which can be used to accumulate As(III) without (under certain concentrations) toxifying E. coli. The expression of both genes is inducible by addition of IPTG.

Notes

The two genes were cloned in such a way that a polycistronic mRNA will be formed, but after translation two separate proteins will be produced as both still contain a stop-codon.

Source

The device is composed from BBa_K190032, which contains the lactose promoter (BBa_R0010) together with fMT ( BBa_K190019) , this is fused with BBa_K190028, GlpF the arsenic transporter.

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_K190019	BBa_K190019
public	BBa_K190028	BBa_K190028

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_K190019	209,430	BBa_K190019
BBa_K190028	437,1282	BBa_K190028

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K190038/1

Member of these Collections

• iGEM Parts Registry