Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eloise Lloyd
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-07 03:40:19
pT7- E. coli optimised - KillerOrange (EOKO)
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1914001_sequence (Version 1) |
Description
KillerOrange is a photoactiated kill switch with an IPTG inducible T7 promoter with E.coli optimised protein coding region of KillerRed mutant.Notes
Codon optimised for E.coli K12 using IDT's online serverSource
Mutant of KillerRed, a red fluorescent protein from jellyfish Anthomedusae sp chromoprotein anm2CP, produced by random mutatagenesis using an error-prone PCR.Sarkisyan, K.S., Zlobovskaya, O.A., Gorbachev, D.A., Bozhanova, N.G., Sharonov, G.V., Staroverov, D.B., Egorov, E.S., Ryabova, A.V., Solntsev, K.M., Mishin, A.S. and Lukyanov, K.A., 2015. KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. PloS one,10(12), p.e0145287.
| Access | Instance | Definition |
| public public public public | BBa_I712074 BBa_B0034 BBa_K1914000 BBa_B0015 | BBa_I712074 BBa_B0034 BBa_K1914000 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I712074 BBa_B0034 BBa_K1914000 BBa_B0015 | 1,46 55,66 73,789 798,926 | BBa_I712074 BBa_B0034 BBa_K1914000 BBa_B0015 |