Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chia-En,Wong
Date created: 2016-09-22 11:00:00
Date modified: 2016-10-14 09:06:20
pBAD-RBS-lysis-B0015
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1920006_sequence (Version 1) |
Description
pBAD is an E.coli promoter that is induced by L-arabinose.In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription [1]. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription [1].[***]However, under the higher concentration of glucose environment, pBAD will be inhibited[***]. But there is still a proper range of glucose concentration, allowing us to control the expression of the lysis.
Notes
For biosafety, we need to precisely control the expression of lysis. So we choose the pBAD which is an promoter induced by L-arabinose.Source
Provided by our advisor, I-Son Ng.| Access | Instance | Definition |
| public public public public | BBa_K206000 BBa_B0034 BBa_K117000 BBa_B0015 | pBAD BBa_B0034 BBa_K117000 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K206000 BBa_B0034 BBa_K117000 BBa_B0015 | 1,130 139,150 157,300 309,437 | pBAD BBa_B0034 BBa_K117000 BBa_B0015 |