BBa_K1926022

BBa_K1926022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1926022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xinyi Liu
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-11 06:31:46

The SNAP-mCHERRY UNIT



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1926022_sequence (Version 1)

Description

This part is the SNAP-mCHERRY UNIT of cyclebow including a SNAP UNIT and a mCHERRY UNIT. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The mCHERRY UNIT, which can be cut off by VIKA, includes a degradation tag named DBOX, a fluorescent gene mCherry and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox.


You may use this part to:

1) Test the cut-off efficiency of recombinase CRE and VIKA;

2) Estimate the time needed for genome repair because mCherry can only be expressed after the cut off of SNAP UNIT and genome repair;

3) Fast cut off this part by transient transfecting recombinase gene into nucleus;

4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company.

Notes

None

Source

The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The sequence of Vox and DBOX was retrieved from Addgene and got through denovo synthesis. mCHERRY was got from commercial plasmid: pentry dcas9 mcherry bira.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1926022/1