Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tomoki Uchino
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 08:34:03
constitutive expression of CBDclos fused to BclA with 6xHis tag
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1933003_sequence (Version 1) |
Description
CBDclos fused to BclA with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBD) fused to surface expression anchoring domain(BclA), connected by a 6xHis tag to be easily identified by Western blotting.For more information, please visit our Wiki page.
Notes
BclA sequence was optimized to expression in E. coli K-12Source
The BclA surface expression component derives from Bacillus anthracis(34F2), it is a hair-like protein that covers B. anthracis??? spores. We used parts BBa_K1933000 from iGEMKyoto 2016 for construction. We outsourced chemical synthesis of this part for construction. CBDclos derives from Clostridium cellulovorans, and is part of a multi-unit cellulosomes. We used parts BBa_K1321341 from iGEM Imperial 2014 for construction.| Access | Instance | Definition |
| public public public public | BBa_J23105 BBa_B0034 BBa_K1933000 BBa_K1321002 | BBa_J23105 BBa_B0034 BclA-His BBa_K1321002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23105 BBa_B0034 BBa_K1933000 BBa_K1321002 | 1,35 36,47 48,137 138,437 | BBa_J23105 BBa_B0034 BclA-His BBa_K1321002 |