Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tomoki Uchino
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 12:04:53
constitutive expression of CBDcex fused to BclA with 6xHis tag
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1933102_sequence (Version 1) |
Description
CBDcex fused to BclA with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBD) fused to surface expression anchoring domain(BclA), connected by a 6xHis tag to be easily identified by Westernblotting. For more information, please visit our Wiki page.Notes
The stop codon for this part is not in the coding region, but in the suffix region.Source
Promoter and RBS:BBa_K1933004The BclA surface expression component derives from Bacillus anthracis(34F2), it is a hair-like protein that covers B. anthracis??? spores. We used parts BBa_K1933000 from iGEMKyoto 2016 for construction. We outsourced chemical synthesis of this part for construction.
CBDcex derives from Cellulomonas fimi, and is part of an exoglucanase secreted by this organism. We used parts BBa_K1321342 from iGEM Imperial 2014 for construction.
| Access | Instance | Definition |
| public public public | BBa_K1933004 BBa_K1933000 BBa_K1321003 | BBa_K1933004 BclA-His BBa_K1321003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1933004 BBa_K1933000 BBa_K1321003 | 1,60 61,144 145,471 | BBa_K1933004 BclA-His BBa_K1321003 |