Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oumnia KARIM
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-17 01:24:23
pNagA-RFP in pSBBs0K-mini plasmid
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1937004_sequence (Version 1) |
Description
(Chassis B. subtilis, carrier plasmid pBS0K)Length: 6843 bp
Background:
pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis.
In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate. This NAG molecule is the major component of chitin, a characteristic molecule of fungi.
This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)
Notes
The part includes the pNagA promoter and its RBS (position 3,595,156 to 3,595,356 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.This part is a subcloning of BBa_K1937003 pNagA part in pSBBS0K_mini plasmid (BBa_K1937001).Source
The part include the pNagA promoter and its RBS (position 3,595,156 to 3,595,356 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.This part is a subcloning of BBa_K1937003 pNagA part in pSBBS0K_mini plasmid (BBa_K1937001).In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate.
| Access | Instance | Definition |
| public public | BBa_K1937003 BBa_K1937001 | BBa_K1937003 BBa_K1937001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1937003 BBa_K1937001 | 1,992 1001,6857 | BBa_K1937003 BBa_K1937001 |