BBa_K1946004

BBa_K1946004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1946004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ayşe Naz Ozant??rk

Date created: 2016-10-18 11:00:00

Date modified: 2016-10-19 10:23:29

Toluene/Xylene Sensor

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1946004_sequence (Version 1)

Description

This part responds to the presence of toluene, m-, p- or o-xylenes and produces a green fluorescent signal. The transcriptional regulatory protein XylR recognizes these organic molecules and activates pu promoter which in turn will lead to the synthesis of sfGFP.

Notes

XylR (BBa_K1946005) in this composite part was codon optimized by us for E.coli K12.

Source

The source of XylR (BBa_K1946005) can be found at GenBank: AAB59162.1 . pu promoter (BBa_K1946006) was synthetically synthesized from GeneWiz based on Pseudomonas putida TOL plasmid. sfGFP sequence can be accessed in the registry at BBa_I746916.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pL(tetO)	3,57	feature/promoter, promoter
RBS	73,77	feature/rbs, ribosome_entry_site
XylR (K1946005)	87,1784	engineered_region, feature/BioBrick
rrnB T1 (B0010)	1788,1892	engineered_region, feature/BioBrick
pu (K1946005)	1899,2220	feature/BioBrick, engineered_region
RBS	2227,2241	feature/rbs, ribosome_entry_site
sfGFP (I746916)	2254,2967	engineered_region, feature/BioBrick
rrnB T1 (B0010)	2977,3081	engineered_region, feature/BioBrick

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1946004/1

Member of these Collections

• //function/sensor
• iGEM Parts Registry