Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jie Tang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-15 10:22:18
This part serves as a catch system expressed in E. coli.
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1947023_sequence (Version 1) |
Description
There is a highly specific recognition and covalent conjugation between Spytag (a peptide with 13 amino acids) and Spycatcher (a small protein consisting of 138 residues). A protein of interest with a Spytag at its N- or C-terminus is expressed in E. coli and present in bacterial lysate.Notes
We add one glycine between Spytag and TEV site to prevent two short peptides from influencing each other.Source
Streptococcus pyogenes fibro-nectin-binding protein FbaB contains a domain with a spontaneous isopeptide bond between Lys31 and Asp117. This domain can be splitted into two fragments: the small one is named as Spytag, while the big one is Spycatcher. Spytag is separated from the second immunoglobulin-like collagen adhesin domain (CnaB2) which is come from the fibronectin binding protein FbaB.| Access | Instance | Definition |
| public public public | BBa_K1159201 BBa_J18918 BBa_K592009 | BBa_K1159201 TEVsite amilCP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1159201 BBa_J18918 BBa_K592009 | 1,39 48,68 75,743 | BBa_K1159201 TEVsite amilCP |