BBa_K1949052

BBa_K1949052 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1949052

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yoshio Takata

Date created: 2016-10-05 11:00:00

Date modified: 2016-10-14 09:13:40

PBAD-rbs-amiE

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1949052_sequence (Version 1)

Description

This gene codes for protein AmiE. AmiE is an acylase that is capable of degrading long chain N-acyl homoserine lactone (AHL) molecules with acyl chains longer than six carbons.

Notes

We applied a site-specific mutation to the amiE in order to destroy a recognition site of EcoRI. We changed the 291th base A to G.

Source

AmiE is derived from artificially synthesized DNA.

Components

Access	Instance	Definition
public	BBa_I0500	pBad/araC
public	BBa_B0034	BBa_B0034
public	BBa_K1949050	BBa_K1949050

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I0500	1,1210	pBad/araC
BBa_B0034	1219,1230	BBa_B0034
BBa_K1949050	1237,2712	BBa_K1949050

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1949052/1

Member of these Collections

• iGEM Parts Registry