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BBa_K195618

BBa_K195618 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K195618

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yun-Hsiang Chang

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:17

pPenI with activator gene CII and pRE with GFP generator

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K195618_sequence (Version 1)

Description

This part is composed of pPenI with inducer gene CII, and it would induce pRE to produce green fluorescent protein.The coding sequence CII could induce BBa_K116603,the regulatory promoter pRE from λ phage. The PenI repressible promoter is repressed by PenI (BBa_C0074).The pRE would be induced by CII produced by the downstream gene of the regulatory promoter pPenI and produce green fluorescent protein.

Notes

none

Source

biobrick

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0010 BBa_B0012 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	PenI BBa_B0034 CII BBa_B0010 BBa_B0012 pRE BBa_B0034 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0010 BBa_B0012 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	1,77 86,97 104,397 406,485 494,534 543,590 599,610 617,1336 1345,1424 1433,1473	PenI BBa_B0034 CII BBa_B0010 BBa_B0012 pRE BBa_B0034 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K195618/1