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BBa_K195623

BBa_K195623 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K195623

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yun-Hsiang Chang

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:17

CII/PenI Damped Oscillator With GFP Reporter (II)

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Details

Types	DnaRegion
Roles	engineered_region Device
Sequences	BBa_K195623_sequence (Version 1)

Description

This part is a time delay device using the regulatory promoter and the interaction between the inducer, repressor and regulatory promoter. The promoter pRE could be induced by BBa_K116602,the inducer CII, which results in the production of the TetR and green fluorescent protein. PenI from Bacillus licheniformis with LVA tail could repress the regulatory promoter BBa_R0074 pPenI, which results in the lower production of the inducer CII.

Notes

none

Source

biobrick

Components

Access	Instance	Definition
public public public public public public public public public public public public	BBa_K116603 BBa_B0034 BBa_C0074 BBa_B0015 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015	pRE BBa_B0034 penI BBa_B0015 PenI BBa_B0034 CII BBa_B0015 pRE BBa_B0034 GFP BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K116603 BBa_B0034 BBa_C0074 BBa_B0015 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015	1,48 57,68 75,522 531,659 668,744 753,764 771,1064 1073,1201 1210,1257 1266,1277 1284,2003 2012,2140	pRE BBa_B0034 penI BBa_B0015 PenI BBa_B0034 CII BBa_B0015 pRE BBa_B0034 GFP BBa_B0015

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K195623/1