Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laetitia Warny
Date created: 2009-08-12 11:00:00
Date modified: 2015-05-08 01:11:17
HfsG + HfsH proteins from Caulobacter crescentus
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K196004_sequence (Version 1) |
Description
Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page [1]. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH [2] is a carbohydrate esterase. Here you have a part made of the Biobricks HfsG (BBa_K196002) and HfsH (BBa_K196003).Notes
As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt [3]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.Source
This sequence comes from Caulobacter crescentus.| Access | Instance | Definition |
| public public | BBa_K196002 BBa_K196003 | HfsG HfsH |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K196002 BBa_K196003 | 1,933 940,1716 | HfsG HfsH |