Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eivind B??e Drejer
Date created: 2016-10-20 11:00:00
Date modified: 2016-10-27 07:18:27
Deoxynucleotide ribonuclease expressing plasmid which cuts off self inhibitory RBS tails
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1970000_sequence (Version 1) |
Description
This plasmid contains two genes that code for two separate deoxynucleotide ribonucleases. These are able to bind to the RBS end of a modified mCherry mRNA. The modified mCherry mRNA has a self inhibitory RBS tail, which prevents binding of the transcript to ribosomes. Cleaveage by one of the deoxynucleotide ribonucleases removes the self inhibitory tail from the RBS, freeing the transcript for translation by ribosomes.Notes
None in particular.Source
De novo DNA| Sequence Annotation | Location | Component / Role(s) |
| lacI promoter reverse transcriptase msDNA misc RNA DNAzyme tetR promoter misc RNA msDNA DNAzyme reverse transcriptase | 1,201 413,1375 283,393 213,294 318,362 1385,1438 1447,1528 1518,1628 1552,1596 1648,2610 | feature/promoter promoter feature/protein CDS feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc promoter feature/promoter feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/protein CDS |