Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuli Liang
Date created: 2016-09-15 11:00:00
Date modified: 2016-09-16 09:23:57
dszBCAD with T7 promoter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1972010_sequence (Version 1) |
Description
We constructed dszBCAD genes with T7 promoter and choose E.coli BL21 as our host cell. When IPTG is added to our system, DszABCD enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway.Notes
No.Source
4 enzyme genes was systhsized respectively and we construted them to one cassette.| Access | Instance | Definition |
| public public public public public public public | BBa_I712074 BBa_J61100 BBa_K1972001 BBa_K1972002 BBa_K1972000 BBa_K1972003 BBa_B0012 | BBa_I712074 BBa_J61100 BBa_K1972001 BBa_K1972002 BBa_K1972000 BBa_K1972003 BBa_B0012 |