Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuli Liang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 08:29:53
dszBAC with tac promoter and dszD with lac promoter
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1972013_sequence (Version 1) |
Description
We constructed dszBCA genes with tac promoter and dszD gene with lac promoter choose E.coli BL21 as our host cell. When IPTG is added to our system, DszABCD enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway.Each single enzyme gene was synthesized by Generay with E.coli optimized and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)
Notes
Not yet.Source
Each single enzyme gene was synthesized by Generay with E.coli optimized and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1972005 BBa_K1972004 BBa_K1972006 LacUV5 promoter double teminator BBa_K1972003 double teminator LacI promoter LacI double teminator | 1,1770 1779,2561 2570,3841 3979,4021 3850,3978 4022,4617 4618,4746 4755,4832 4956,5919 5924,6052 | feature/BioBrick engineered_region engineered_region feature/BioBrick engineered_region feature/BioBrick promoter feature/promoter feature/misc sequence_feature engineered_region feature/BioBrick sequence_feature feature/misc feature/promoter promoter feature/cds CDS feature/misc sequence_feature |