BBa_K1973003

BBa_K1973003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1973003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alvaro Escobar Doncel
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 05:24:24

nahR-Psal-lapG



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1973003_sequence (Version 1)

Description

nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. lapG (see BBa_K1973000) is expressed from the Psal promoter in the presence of this molecule.

Notes

We digested BBa_K1973000 as a vector (EcoRI and XbaI) and BBa_K1973033 as an insert (EcoRI and SpeI), and then we ligate them.

Source

Molecular cloning. We use the parts BBa_K1973033 and BBa_K1973000 to generate this part

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1973003/1