Types | DnaRegion
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Roles | CDS
Coding
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Sequences | BBa_K1973003_sequence (Version 1)
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Description
nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. lapG (see BBa_K1973000) is expressed from the Psal promoter in the presence of this molecule.
Notes
We digested BBa_K1973000 as a vector (EcoRI and XbaI) and BBa_K1973033 as an insert (EcoRI and SpeI), and then we ligate them.
Source
Molecular cloning. We use the parts BBa_K1973033 and BBa_K1973000 to generate this part