BBa_K1973004

BBa_K1973004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1973004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alvaro Escobar Doncel
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 05:38:46

nahR-Psal-yhjH



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1973004_sequence (Version 1)

Description

nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. yhjH (see BBa_K777121) is expressed from the Psal promoter in the presence of this molecule.

Notes

We digested BBa_K1973034 as a vector (EcoRI and XbaI) and BBa_K1973033 as an insert (EcoRI and SpeI), and then we ligate them.

Source

Molecular cloning.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1973004/1