BBa_K1973005

BBa_K1973005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1973005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alvaro Escobar Doncel

Date created: 2016-10-10 11:00:00

Date modified: 2016-10-11 05:51:56

nahR-Psal-nasF

Details

• Types: DnaRegion
• Roles: engineered_region; Intermediate
• Sequences: BBa_K1973005_sequence (Version 1)

Description

nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter.

Notes

We digested BBa_K1973001 as a vector (EcoRI and XbaI) and BBa_K1973033 as an insert (EcoRI and SpeI), and then we ligate them.

Source

Molecular cloning.

Components

Access	Instance	Definition
public	BBa_K1973033	BBa_K1973033
public	BBa_K1973001	BBa_K1973001

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1973033	1,1039	BBa_K1973033
BBa_K1973001	1048,1151	BBa_K1973001

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1973005/1

Member of these Collections

• iGEM Parts Registry