BBa_K1973007

BBa_K1973007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1973007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alvaro Escobar Doncel

Date created: 2016-10-10 11:00:00

Date modified: 2016-10-11 05:59:00

nahR-Psal-nasF-lapG

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1973007_sequence (Version 1)

Description

nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. lapG (see BBa_K1973000) is expressed from the Psal promoter in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter.

Notes

We digested BBa_K1973000 as a vector (EcoRI and XbaI) and BBa_K1973005 as an insert (EcoRI and SpeI), and then we ligate them.

Source

Molecular cloning.

Components

Access	Instance	Definition
public	BBa_K1973005	BBa_K1973005
public	BBa_K1973000	BBa_K1973000

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1973005	1,1151	BBa_K1973005
BBa_K1973000	1160,1822	BBa_K1973000

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1973007/1

Member of these Collections

• iGEM Parts Registry