Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alvaro Escobar Doncel
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 03:34:58
nahR-Psal-nasF-yhjH
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1973008_sequence (Version 1) |
Description
nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. yhjH (see BBa_K1973034) is expressed from the Psal promoter in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter.Notes
We digested BBa_K1973034 as a vector (EcoRI and XbaI) and BBa_K1973005 as an insert (EcoRI and SpeI), and then we ligate them.Source
Molecular cloning.| Access | Instance | Definition |
| public public | BBa_K1973005 BBa_K1973034 | BBa_K1973005 BBa_K1973034 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1973005 BBa_K1973034 | 1,1151 1160,1948 | BBa_K1973005 BBa_K1973034 |