Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alvaro Escobar Doncel
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 03:58:52
nahR-Psal-nasF-xylS2
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1973017_sequence (Version 1) |
Description
nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. xylS2 (BBa_K1973002) codifies the modified XylS2 protein from Pseudomonas putida MT1, which is a transcriptional activator of the Pm promoter in response to salicylate. This module provides a tight expression regulation by salicylate thanks to a two-step (NahR and XylS2) system.Notes
We digested BBa_K1973002 as a vector (EcoRI and XbaI) and BBa_K1973005 as an insert (EcoRI and SpeI), and then we ligate them.Source
Molecular cloning.| Access | Instance | Definition |
| public public | BBa_K1973005 BBa_K1973002 | BBa_K1973005 BBa_K1973002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1973005 BBa_K1973002 | 1,1151 1160,2146 | BBa_K1973005 BBa_K1973002 |