BBa_K1973020

BBa_K1973020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1973020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alvaro Escobar Doncel
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 03:58:27

nahR-Psal-xylS2



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1973020_sequence (Version 1)

Description

nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. xylS2 (BBa_K1973002) is expressed from the Psal promoter in the presence of this molecule. This module provides a tight expression regulation by salicylate thanks to a two-step (NahR and XylS2) system.

Notes

We digested BBa_K1973002 as a vector (EcoRI and XbaI) and BBa_K1973005 as an insert (EcoRI and SpeI), and then we ligate them.

Source

Molecular cloning.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1973020/1