BBa_K1973020

BBa_K1973020 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1973020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alvaro Escobar Doncel

Date created: 2016-10-11 11:00:00

Date modified: 2016-10-12 03:58:27

nahR-Psal-xylS2

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1973020_sequence (Version 1)

Description

nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. xylS2 (BBa_K1973002) is expressed from the Psal promoter in the presence of this molecule. This module provides a tight expression regulation by salicylate thanks to a two-step (NahR and XylS2) system.

Notes

We digested BBa_K1973002 as a vector (EcoRI and XbaI) and BBa_K1973005 as an insert (EcoRI and SpeI), and then we ligate them.

Source

Molecular cloning.

Components

Access	Instance	Definition
public	BBa_K1973033	BBa_K1973033
public	BBa_K1973002	BBa_K1973002

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1973033	1,1039	BBa_K1973033
BBa_K1973002	1048,2034	BBa_K1973002

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1973020/1

Member of these Collections

• iGEM Parts Registry