Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jos?? Mart??n G??mez
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 03:59:03
miniTn7BBGm-nahR-Psal-pleD*-glpF
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1973038_sequence (Version 1) |
Description
nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. pleD* (BBa_K1973016) and glpF (BBa_K1973026) are expressed from the Psal promoter in the presence of this molecule. Therefore, this construct encodes the genes that are necessary for biofilm induction and glycerol uptake, connecting both activities under the same expression and regulation system. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).Notes
This composite part was created by cloning the parts BBa_K510000, BBa_K1973033, BBa_K1973001, BBa_K1973016 and BBa_K1973026 together.Source
This composite part was created by cloning the parts BBa_K510000, BBa_K1973033, BBa_K1973001, BBa_K1973016 and BBa_K1973026 together.| Access | Instance | Definition |
| public public public public public | BBa_K510000 BBa_K1973033 BBa_K1973001 BBa_K1973016 BBa_K1973026 | BBa_K510000 BBa_K1973033 BBa_K1973001 BBa_K1973016 BBa_K1973026 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K510000 BBa_K1973033 BBa_K1973001 BBa_K1973016 BBa_K1973026 | 1,4388 4397,5435 5444,5547 5556,6940 6949,7809 | BBa_K510000 BBa_K1973033 BBa_K1973001 BBa_K1973016 BBa_K1973026 |