Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MA Xinyi
Date created: 2016-08-25 11:00:00
Date modified: 2016-08-26 02:45:54
PBP5 coding device (promoter+PBP5+T7 terminator)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1979003_sequence (Version 1) |
Description
This device codes for Penicillin binding protein 5. The coding sequence is tagged by 6??his and T7, and it is controlled by a constitutive promoter BBa_J23101 and a T7 terminator.Notes
Histidine tag is added for affinity purification to harvest the proteins.Source
The PBP5 and terminator sequences are retrieved form GenBank, and the promoter sequence comes from the part BBa_J23101.| Sequence Annotation | Location | Component / Role(s) |
| J23101 g10-L rbs 6??his tag T7 tag start codon T7 Terminator PBP5 | 1,35 58,64 84,101 131,164 173,176 1476,1523 177,1385 | feature/promoter promoter feature/rbs ribosome_entry_site tag feature/tag feature/tag tag start_codon feature/start sequence_feature feature/misc feature/protein CDS |