BBa_K1979004

BBa_K1979004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1979004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MA Xinyi
Date created: 2016-08-27 11:00:00
Date modified: 2016-08-28 02:50:06

GFP-PBP5



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_K1979004_sequence (Version 1)

Description

This device codes for the GFP-PBP5 fusion protein.

Notes

Histidine tag is added for affinity purification to harvest the proteins. g10-L is a strong RBS in E. coli but also well suited for foreign gene expression.The effect of the ribosome binding site (RBS) on in vitro translation has been investigated extensively (Karig et al. 2012; Lentini et al. 2013; Takahashi et al. 2013). Most important for an efficient reaction is a defined distance (4-9 bases) of the RBS to the following start codon (Karig et al. 2012; Lentini et al. 2013), but the strength of the RBS has a minor effect as long as it is not too weak (Karig et al. 2012; Takahashi et al. 2013).

Source

The GFP and PBP5 sequences are retrieved from the GenBank, and the promoter comes from J23101.

Sequence Annotation Location Component / Role(s)
J23101
g10-L rbs
his
T7
GFP
PBP5
T7 terminator
1,34
55,62
84,101
132,164
174,890
891,2099
2190,2237
promoter feature/promoter
ribosome_entry_site feature/rbs
tag feature/tag
tag feature/tag
feature/protein CDS
CDS feature/protein
stop_codon feature/stop
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1979004/1