| Types | DnaRegion
|
| Roles | Generator
engineered_region
|
| Sequences | BBa_K1979004_sequence (Version 1)
|
Description
This device codes for the GFP-PBP5 fusion protein.
Notes
Histidine tag is added for affinity purification to harvest the proteins. g10-L is a strong RBS in E. coli but also well suited for foreign gene expression.The effect of the ribosome binding site (RBS) on in vitro translation has been investigated extensively (Karig et al. 2012; Lentini et al. 2013; Takahashi et al. 2013). Most important for an efficient reaction is a defined distance (4-9 bases) of the RBS to the following start codon (Karig et al. 2012; Lentini et al. 2013), but the strength of the RBS has a minor effect as long as it is not too weak (Karig et al. 2012; Takahashi et al. 2013).
Source
The GFP and PBP5 sequences are retrieved from the GenBank, and the promoter comes from J23101.
