BBa_K1987000

BBa_K1987000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1987000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Caroline Blassick

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-29 11:18:34

GFP with BsaI sites for promoter insertion

Details

• Types: DnaRegion
• Roles: Measurement; engineered_region
• Sequences: BBa_K1987000_sequence (Version 1)

Description

This testing construct consists of a degradation-tagged GFP orf preceded by the lac promoter and RBS. The promoter and RBS are flanked by BsaI sites, allowing for different promoter+RBS combinations to be easily swapped into the construct. The strength and expression profile of the promoter/RBS combo can then be easily measured.

Notes

BsaI site was removed from the GFP orf through a silent point mutation.

Source

Largely based off of part

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034
public	BBa_K1212006	BBa_K1212006
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0034	209,220	BBa_B0034
BBa_K1212006	229,1280	BBa_K1212006
BBa_B0015	1289,1417	BBa_B0015

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1987000/1

Member of these Collections

• iGEM Parts Registry