Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Clifton Davis
Date created: 2009-10-22 11:00:00
Date modified: 2015-05-08 01:11:19
CUACC with RFP Reporter
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K199098_sequence (Version 1) |
Description
Suppressor tRNA expressed by the promoter, plac, upstream of RFP with a 5-bp insertion, CUACC. The tRNA is designed to suppress the insertion, allowing the appropriate translation of functional RFP.Notes
N/ASource
From the BioBrick Parts Registry. The tRNA was constructed by oligo assembly, and mutated the RFP reporter, E1010, to add the 5-bp insertion by PCR| Access | Instance | Definition |
| public public public public public | BBa_R0010 BBa_K199045 BBa_R0010 BBa_B0034 BBa_K199075 | LacI BBa_K199045 LacI BBa_B0034 BBa_K199075 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_K199045 BBa_R0010 BBa_B0034 BBa_K199075 | 1,200 209,351 360,559 568,579 588,1316 | LacI BBa_K199045 LacI BBa_B0034 BBa_K199075 |