Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shilo Ohayon
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-18 03:40:03
PctA-Tar GFP tagged expression system (promoter+RBS+coding+terminator)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1992010_sequence (Version 1) |
Description
IntroductionA GFP gene was fused to the PctA chimera. The plasmid is comprised of a promoter, a strong RBS, the chimera, a GFP gene and a terminator. In addition, a proper linker ought to be introduced in order to ensure the right correct fold of the protein.
Usage and Biology
This device was used as a proof of concept in order to verify the migration of the new chemoreceptor to the poles of the bacterial membrane.
Design Considerations
A flexible linker was introduced to fuse the GFP gene to the chimera. The linker is essential for the protein to fold in the right manner and for the correct tertiary structure to be obtained.
For methods and results please go to PctA-Tar (K1992001)
Notes
noneSource
none| Access | Instance | Definition |
| public public public public public public | BBa_J23100 BBa_B0034 BBa_K1992001 BBa_J18921 BBa_E0040 BBa_B0015 | BBa_J23100 BBa_B0034 BBa_K1992001 3xGS GFP BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K1992001 BBa_J18921 BBa_E0040 BBa_B0015 | 1,35 44,55 62,1981 1990,2007 2014,2733 2742,2870 | BBa_J23100 BBa_B0034 BBa_K1992001 3xGS GFP BBa_B0015 |