BBa_K1997017

BBa_K1997017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1997017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chushu Zhu

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-13 06:51:33

P+R->sGFP-N->FRB->RBS->FKBP ->sGFP-C->TER

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1997017_sequence (Version 1)

Description

This part is an integrated tool for protein-protein interaction research using split-GFP system as reporter. the "FRB-RBS-FKBP" subpart can be easily replaced using Golden Gate technique with BsaI

Notes

We added BsaI sites at the flank of "FRB-RBS-FKBP" sequence so that this sequence can be easily replaced

Source

This part was synthesized by IDT

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1789012	1,223	engineered_region, feature/BioBrick
barcode	224,228	engineered_tag, feature/barcode
sGFP-N	229,699	feature/cds, CDS
10aa linker	700,729	CDS, feature/protein
FRB	742,1026	feature/cds, CDS
RBS	1027,1056	ribosome_entry_site, feature/rbs
FKBP	1057,1380	CDS, feature/cds
10aa linker	1393,1422	feature/protein, CDS
sGFP-C	1423,1668	feature/cds, CDS
barcode	1669,1674	engineered_tag, feature/barcode
BBa_B0015	1675,1803	feature/BioBrick, engineered_region

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1997017/1

Member of these Collections

• iGEM Parts Registry