Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: William Cho
Date created: 2016-06-16 11:00:00
Date modified: 2016-10-02 01:25:32
PETase (PET-degrading enzyme, origin I. sakaiensis)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2010000_sequence (Version 1) |
Description
PETase is the PET-degrading enzyme first identified in Ideonella sakaiensis.This sequence is the E. coli K12 optimized DNA sequence for PETase.
Notes
This sequence was optimized for E. coli K12 using the Codon Optimization Tool from the IDT website.Source
This part comes from Ideonella sakaiensis, identified in "A bacterium that degrades and assimilates poly(ethylene terephthalate)" (Yoshida et al. 2016).| Sequence Annotation | Location | Component / Role(s) |
| PETase T7 Promoter RBS 34 GGS Linker His-Tag Stop Codon Start Codon | 59,928 1,46 47,58 929,964 965,982 983,988 59,61 | feature/protein CDS feature/promoter promoter feature/rbs ribosome_entry_site sequence_feature feature/misc tag feature/tag stop_codon feature/stop start_codon feature/start |